Plasmid Involvement in Parathion Hydrolysis by Pseudomonas diminuta
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چکیده
منابع مشابه
Limits of diffusion in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta.
The catalytic mechanism for the enzymatic hydrolysis of a series of paraoxon analogues by the phosphotriesterase from Pseudomonas diminuta has been determined. The Brønsted plots relating the pKa of the leaving group to the observed kinetic parameters, Vmax and V/Km, are both nonlinear. This observation is consistent with a change in the rate-limiting step from chemical to physical events as th...
متن کاملStructure-activity relationships in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta.
The mechanism and substrate specificity of the phosphotriesterase from Pseudomonas diminuta have been examined. The enzyme hydrolyzes a large number of phosphotriester substrates in addition to paraoxon (diethyl p-nitrophenyl phosphate) and its thiophosphate analogue, parathion. The two ethyl groups in paraoxon can be changed to propyl and butyl groups, but the maximal velocity and Km values de...
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A bacterial strain was isolated from a wastewater lagoon and identified as Pseudomonas fluorescens. This isolate was able to utilize linalool as a sole carbon and energy source. The ability was found to be encoded on a 60-megadalton transmissible plasmid, pSRQ60. The plasmid was also mated into a commercial waste treatment strain, which expanded its ability to utilize other isoprenoid compounds.
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Pseudomonas diminuta, P. putida and P. aeruginosa were established as a biodegrading agent for Methyl Parathion (MP) at 1200 mg/L, under basic growth parameters for mesophiles. In this study, the effects of process parameters, such as temperature, pH, salinity, carbon and nitrogen sources on the growth of those bacteria were studied and optimized to establish higher degradation of the target ch...
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Three naturally occurring toxigenic strains (HB-36, G-50, and HB-33), one nontoxigenic strain (HB-20), and one ultraviolet light-induced toxinless mutant (G-50 Tox) of Pseudomonas phaseolicola were examined by dye-buoyant density equilibrium centrifugation for the presence of plasmid deoxyribonucleic acid. All strains contained plasmid deoxyribonucleic acid. Comparison of the plasmid deoxyribon...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1982
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.44.1.246-249.1982